| 摘要 |
PURPOSE:To achieve stable coloration, by containing phenylenediamine or a derivative thereof, an org. reducing agent and a water soluble high-molecular compound. CONSTITUTION:o-Phenylenediamine is used as phenylenediamine or a derivative thereof, oxalic acid or a metal salt thereof is used as an org. reducing agent and polyethylene glycol is used as a water soluble high-molecular compound while the addition amount of phenylenediamine or the derivative thereof is set to 1- 100mM, that of the org. reducing agent to 0.1mM-5M and that of the water soluble high-molecular compound to 0.001-10wt%, and enzymatic reaction is performed in a light or dark place in a pH range of 4-9 at a reaction temp. of 2-60 deg.C. Then, absorbancy of the formed coloration substance is measured at the wavelength of an absorption max. value and compared with a preliminarily formed calibration curve to measure hydrogen peroxide or the activity of peroxidase in specimen water. |
