| 摘要 |
<p>PURPOSE:To introduce regeneration of plant, by cultivating a protoplast of a plant cell in a thin film of culture solution. CONSTITUTION:The surface of culture container is coated with a thin layer of a hydrophilic substrate, a layer of culture solution having 100-400mum is formed on the surface of the substrate, a protoplast is cultivated in the thin layer of the culture solution. A hydrophilic high polymer substance such as agar, alginic acid, gelatin, etc., is preferably used as the substrate. Medium MS, medium B5, medium N6, medium R2, etc. are used as the culture solution depending upon properties of the protoplast handled. Better results are obtained by the substrate layer with <=1mm, especially <=200mum.</p> |
