| 摘要 |
PURPOSE:To measure accurately pepsin in a sample, by preparing the sample with a pepsinogen activating reagent, adding a protein substrate thereto and determining the change in amount of acid-soluble protein in the sample. CONSTITUTION:A pepsinogen activating reagent, e.g. hydrochloric acid, sulfuric acid or phosphoric acid, is added to a sample, e.g. gastric juice or urine, to convert the pepsinogen into pepsin. A substrate protein, e.g. blood serum, is added thereto to initiate pepsin enzymic reaction and form an acid-soluble protein, which is then measured. The pepsinogen or pepsin is measured from the change in amount of the acid-soluble protein before and after the addition of the substrate protein. The amount of the acid-soluble protein is measured by adding an acidic substance to the sample, centrifuging the resultant mixture and measuring the resultant protein according to the conventional method.
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