| 摘要 |
PURPOSE:To provide the titled reagent capable of determining amylase activity in the state free of intrinsic glucose and extrinsic maltose, by using a phosphoglucose isomerase and phosphofructokinase in combination with a phosphoric acid ester of a sugar (acid). CONSTITUTION:The intrinsic glucose and extrinsic maltose existing in high concentrations in a specimen are converted to a material inert to the amylase activity within 2min by the first reagent composed mainly of alpha-glucosidase or maltose phosphorylase, glucokinase, phosphoglucose isomerase (abbreviated as PGI), phosphofructokinase and adenosine 5'-triphosphate. The treated specimen is added with the second reagent composed mainly of an oligosaccharide and a phosphoric acid ester of a sugar (acid) to stop the PGI activity instantaneously and to enable the determination of amylase activity in body fluid.
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