| 摘要 |
<p>Disclosed is a reagent for measuring amylase activity in body fluids by cleaving an oligosaccharide having a defined chain length with amylase in body fluids to produce a glucose and measuring said glucose, characterized in that the reagent is divided to two portions, the first portion comprises an enzyme for converting a glucose and/or maltose naturally present in body fluids to glucose-6-phosphate, phosphoglucose isomerase, phosphofructokinase and adenosine-5'-triphosphate, and the second portion comprises said oligosaccharide being used as a substrate and a phosphoric acid ester of saccharides and/or saccharic acids; and a method for measuring amylase activity in body fluids comprising;</p><p>eliminating glucose and/or maltose naturally present in body fluids with a first reagent comprising an enzyme for converting the glucose and/or maltose to glucose-6-phosphate, phosphoglucose isomerase, phosphofructokinase and adenosine-5'-triphosphate,</p><p>adding a second reagent comprising a oligosaccharide substrate and a phosphoric acid ester of saccharides and saccharic acids to eliminate the phosphoglucose isomerase activity and to convertthe oligosaccharide fragments formed by the action of amylase on body fluids to glucose by means of alpha-glucosidase or maltose phosphorylase, and</p><p>measuring the amount of the obtained glucose. The reagent of the present invention fully eliminates the interruption of endogeneous glucose or exogeneous maltose with the measurement method of amylase activity to provide an improved accuracy of measuring amylase activity.</p> |
