| 摘要 |
PURPOSE:To make possible automatic biochemical analysis by adjusting monomer type IgG and polymer type IgG to one to 0.7 or above as modified human gamma-globulin and using the same as an antigen in measuring the RF in the human serum. CONSTITUTION:The human gamma-globulin is dissolved to 1% concn. in 0.02M HEPES buffer soln. (7.2phipH) contg. NaN3 at 0.1% concn. and NaCl at 0.8% concn. and the soln. is treated for 30min at 53-63 deg.C, by which the soln. is thermally modified. The light absorptivity of water blank is first measured by using an automatic biochemical analyzing instrument, then the absorptivity of the specimen serum S and the 1st reagent R1 consisting of NaCl, NaN3, chondoloitin sulfuric acid, TX-100 and polyethylene glycol is measured for 5min; further the 2nd reagent R2 consisting of the modified human gamma-globulin subjected to the above-mentioned thermal modification is added to the soln. and the absorptivity thereof is measured.
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