| 摘要 |
PURPOSE:To measure exactly a vital material with ammonia or urea as the resulted product of reaction by consuming the ammonia which exists already in a specimen then adding ATP or/and chelate agent to stop iCDH reaction, adding enzyme to form ammonia to the specimen and measuring the formed ammonia. CONSTITUTION:GlDH, alpha-KG, NADH, isocitric acid, metallic ions such as magnesium ions or manganese ions and iCDH are added and mixed to and with the specimen to consume the ammonia which exists already in the specimen. The ATP or/and chelate agent are then added to the specimen to stop the iCD reaction. The enzyme to form ammonia as the resulted product of reaction is added simultaneously with or after said stop and the formed ammonia is measured. The change from the formula I the formula II is made by the addition of the ATP or/and chelate agent. The reaction of the enzyme to form ammonia is exemplified by the formula III.
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