发明名称 |
Reagents containing an anti-ligand bound to an anti-enzyme and methods for employing said reagents in an immunoassy |
摘要 |
The invention relates to a method and novel reagents for detecting a ligand in a biological sample. In the method, the sample is combined with (i) an enzyme or an enzyme bound to a first macromolecular compound, (ii) substrate for the enzyme, and (iii) an antibody complex comprising anti-ligand bound to anti-enzyme and a second macromolecular compound bound to either one or both of the anti-ligand and anti-enzyme. The activity of the enzyme varies as a function of the unknown amount of ligand contained in the biological sample.
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申请公布号 |
US4621048(A) |
申请公布日期 |
1986.11.04 |
申请号 |
US19840588682 |
申请日期 |
1984.03.12 |
申请人 |
FUJIREBIO KABUSHIKI KAISHA |
发明人 |
ASHIHARA, YOSHIHIRO;SUZUKI, HIROMASA;KASAHARA, YASUSHI |
分类号 |
G01N33/53;G01N33/536;G01N33/542;(IPC1-7):G01N33/536 |
主分类号 |
G01N33/53 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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