| 摘要 |
PCT No. PCT/GB86/00121 Sec. 371 Date Nov. 17, 1986 Sec. 102(e) Date Nov. 17, 1986 PCT Filed Mar. 6, 1986 PCT Pub. No. WO86/05190 PCT Pub. Date Sep. 12, 1986.Efficient precipitation and removal of the proteins fibrinogen and fibronectin from blood plasma fractions, expecially cryoprecipitate, while leaving high yields of blood coagulation factor VIII in the supernatant. This is achieved by the addition of at least 0.15 mg, preferably 0.3-0.9 mg, of a sulphated polysaccharide, especially heparin, per ml of buffered plasma fraction solution while maintaining the temperature of the solution during fibrinogen/fibronectin removal at more than 15 DEG C., preferably 20 DEG -35 DEG C. Lyophilised factor VIII preparation prepared from the factor VIII-rich supernatant product of the invention are suitable for heat treatment at, for example, 70 DEG C. for 24 hours to inactivate blood-born viruses without significant generation of insoluble denatured protein by-products. |
