| 摘要 |
PURPOSE:To obtain a highly sensitive CRP measurement method, by differentiating the kind of animal to be immunized for obtaining a solid-phase antibody and a labelled antibody. CONSTITUTION:An anti-CRP rabbit IgG is used as solid phase antibody while an anti-CRP goat IgG as labelled antibody to react with a sample as measuring antigen (CRP serum) and then, the activity of the labelled substance involving the antigen-antibody reaction is measured to assay CRP. When a 1-step method is applied, a washing (b/f separation) is done after the simultaneous reaction of the solid-phase antibody and the labelled antibody with the measuring antigen (CRP serum sample) and then, the measurement of the activity of the labelled substance involving the antigen-antibody reaction. When a 2-step method is applied, initially, the solid-phase antibody is made to react with the measuring antigen and after a washing, the labelled antibody is added. Following a washing is done again, the activity of the labelled substance is measured.
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