Analytical compositions, elements and methods utilizing reduction of ferric ion chelates to form detectable dyes.

摘要

@ Ferric ion chelates are useful in analytical compositions, elements and methods for the determination of reductants, or other analytes which can react to produce a reductant. The ferric ion chelate is reduced to ferrous ion and ferric ion coordinating ligand. The ferrous ions then coordinate with a suitable ferrous ion coordinating ligand to form a colored complex. In particular, these compositions and elements are useful for detecting nicotinamide adenine dinucleotide, reduced form or nicotinamide adenine dinucleotide phosphate, reduced form, or an analyte (e.g. an enzyme) which produces the reduced forms of nucleotide in biological fluids.