| 摘要 |
Characteristics of two items in a sample are determined by sequentially adding first and second reagents and optically measuring characteristics of the first and second reaction solutions so obtained. For example, transparent containers (2) are supported on a rotatable disc (1). A fixed volume of serum is introduced into one of the containers (2) by means of a pipette (9). The first reagent solution containing alpha -ketoglutaric acid, L-aspartic acid and NADH is introduced by the pump (9) so that an enzyme reaction caused by GOT in the serum proceeds. The container (2) is passed a plurality of times across an optical path (13), and variation with time of difference between absorbance at 340 nm and 376 nm is measured to determine a reaction rate for the first reaction solution. Thereafter, the second reagent solution containing L-alanine is introduced by the pump (10) so that an enzyme reaction caused by GPT proceeds, and the reaction rate of this second reaction solution is determined in the same manner. Activity of GOT in the sample is determined from the reaction rate of the first reaction solution, and the sum of activities of GOT and GPT is determined from the reaction rate of the second reaction solution. |
