发明名称 |
Process for recovering glucagon from pancreas glands |
摘要 |
There is disclosed herein a process for the purification of glucagon from a glucagon salt cake containing pancreas gland-sourced glucagon, which comprises (1) dissolving said glucagon salt cake in an aqueous medium containing a water-miscible organic solvent; (2) contacting the glucagon salt cake solution with a hydrophobic adsorption support at a pH less than about 4 or greater than about 7.5 and at conditions under which the glucagon is adsorbed on said hydrophobic adsorption support; (3) eluting glucagon from said hydrophobic adsorption support with an aqueous solution containing a water-miscible organic solvent to obtain a first glucagon-containing solution; (4) contacting a solution containing glucagon eluted from said hydrophobic adsorption support, said solution having a pH of from about 7.5 to about 10.5, with an anion exchange support at conditions under which the glucagon is adsorbed on said anion exchange support; (5) eluting glucagon from said anion exchange support to obtain a second glucagon-containing solution; and (6) recovering glucagon having increased purity.
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申请公布号 |
US4617376(A) |
申请公布日期 |
1986.10.14 |
申请号 |
US19860833310 |
申请日期 |
1986.02.24 |
申请人 |
ELI LILLY AND COMPANY |
发明人 |
MASKALICK, DAVID G.;ANDERSON, MARIE T. |
分类号 |
C07K14/605;(IPC1-7):C07K7/34 |
主分类号 |
C07K14/605 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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