MEASURING METHOD FOR PERMEABILITY AND DIFFUSION CONSTANT OF FILM

摘要

PURPOSE:To measure rapidly, easily and accurately the permeability of the film for a special chemical species in the aqueous solution including many components such as a fermentation liquid and a body fluid by obtaining the ratio of the time change of the response electric current output of the enzyme electrode and estimating the ratio of the time change of the density of the chemical type to transmit the film. CONSTITUTION:Through a film 1 to measure the permeability and the diffusion constant for a certain chemical species two aqueous solution phases 2 and 3 exist. Into the aqueous solution phase 3, the enzyme electrode is inserted in which a film 5 formed by fixing the oxidizing enzyme with the above- mentioned chemical species as one of the substrates at the surface of the enzyme electrode and the hydrogen peroxide electrode 4. The output electric current from the enzyme electrode is measured by an ammeter 6 and recorded by a recorder 7.