ENZYME-LINKED IMMUNOASSAY, ASSAY KITS AND PAIR-BINDING COMPOUNDS

摘要

An immunoassay, for measuring the amount of a ligand present in a medium, uses a luciferase-labelled binding pair member selected from a ligand and a receptor, in which the luciferase is linked to the binding pair member via its reactive sulfhydryl group and is thereby reversibly inactivated. …A kit, for use in an immunoassay to determine the presence of a ligand in a medium, which comprises a plurality of containers, a first container containing luciferase-labelled receptor or ligand (a purified version of the ligand to be assayed) and a second container containing receptor which may be immobilised. …New compounds have a reactive group which can react to cause reversible inactivation of luciferase and a group which can react with one or more sites on a binding receptor or ligand.