| 摘要 |
<p>Cells which produce EPF are grown in a culture medium to produce a supernatant medium containing the EPF. To purify the EPF, the EPF is absorbed by a selective absorbent in a column, dialysed against a buffer solution, concentrated and gel-filtered. Selected fractions of the filtrate undergo reversed phase high performance liquid chromotography and the purified EPF is eluted from the chromotography column. Monoclonal antibodies to EPF can be produced to detect the presence of EPF in serum and provide a means for detecting pregnancy in female mammals.</p> |
