| 摘要 |
<p>PURPOSE:To provide the titled medium having excellent cell-proliferation property, and suitable for the proliferation of animal cell to produce a valuable material such as interferon, hormone, monoclonal antibody, etc., and composed mainly of a liquid component extracted from the embryo of a fertilized egg. CONSTITUTION:After sterilizing the surface of the shell, a fertilized egg of e.g. chicken is incubated in an incubator at 38-39 deg.C and 85-95% RH for 10-11 days. The embryo is separated from the incubated egg, washed with water, added with clean water or dilute aqueous solution of NaCl, homogenized with a homogenizer in the form of paste, and filtered with a filter such as a filter press or centrifuged to collect a liquid component. The liquid is again filtered with a micro-filter to remove microorganisms, etc., and the filtrate is stored as a medium for cell culture at <=-30 deg.C.</p> |
