ENZYME IMMUNOASSAY FOR ERYTHROPOIETIN

摘要

PURPOSE:To measure the enzyme immunity of erythropoietin by forming an antibody-erythropoietin-labeling material composite on an antibody-conjugated insoluble base then measuring the quantity of the labeling material conjugated onto the base. CONSTITUTION:The antibody-conjugated insoluble base formed by conjugating the antibody reacting specifically with erythropoietin (EPO) on the insoluble base and a specimen liquid contg. the EPO are brought into reaction to conjugate the EPO onto the antibody-conjugated insoluble base. The same is then brought into reaction with the labeling material labeling the antibody reacting specifically with the EPO with enzyme by which the antibody-EPO-labeling material composite is formed on the antibody-conjugated insoluble base. The quantity of the labeling material conjugated on the base is then measured or the quantity of the liberated labeling material which is not conjugated is measured. The quick enzyme immunomeasurement of the EPO in an ordinary inspection room at a low cost with high sensitivity is thus made possible.