摘要 |
PURPOSE:To enable the operation of fractioning quantity of target material in a short time, by dividing the hollow of a column vessel into a grouping of paths about 7-50mm<2> each hollow-crossed area so arranged parallel along the axis of the column to allow the filling of hydroxyapatite (HA) crystals with the plane faces thereof oriented roughly parallel to the direction of the paths. CONSTITUTION:The hollow of a column vessel 1 is divided into a grouping of small area crossed paths about 7-50mm<2> each hollow crossed area so arranged roughly parallel along the axis of the column vessel 1 so that a group construction 7 having small area crossed paths 7a can be inserted into the column vessel 1. When a suspension of HA crystals 6 is introduced into each of the paths 7a in laminar flows, the HA crystals 6 are filled and treated with the plane faces thereof oriented roughly parallel to the direction of the paths to obtain a fractioning column A for chromatography. The average particle diameter of the HA crystals 6 to be filled is preferably about 30-60mum in terms of the diameter of Stokes' equivalent ball. |