DETERMINATION OF CLINICAL PARAMETERS BY ENZYME IMMUNOPROCESS

摘要

A method and a device for the determination of clinical parameters in biological liquids by enzyme immunoprocess, wherein the biological liquid is contacted sequentially with: a) enzyme labelled antibodies or antigens that are absorbed on a solid support in a reversible manner; b) antibodies, antigens, anti-antibodies or antigen-antibody complexes that are immobilized on a solid support in a quantity stoichiometrically equivalent to the enzyme-labelled antibodies or antigens; c) one or more chromogen substrates capable of producing evidencing reaction with the antibody or antigen-conjugated enzymes.