PRODUCTION OF IMMOBILIZED LIVING BODY CATALYST

摘要

PURPOSE:A specific platinum base is treated with an aldehyde, then bonded to a biocatalyst whereby the titled biocatalyst with high activity is obtained regardless of kinds of biocatalysts to be immobilized. CONSTITUTION:A platinum base such as a platinum plate is treated with aminosilane to provide amino groups on the base 1 and coated with a film- forming solution containing albumin and glutar aldehyde as a crosslinking agent to form a crosslinked albumin film 3. Then, the film is coated with a solution containing a polyfunctional aldehyde such as glutar aldehyde to provide aldehyde groups on the crosslinked film 3, washed with a buffer solution of 7.5pH, then the solution is removed and the coated film is dried by standing. The coated film 3 is coated with a solution containing enzymes or microorganisms such as uricase to allow the aldehyde group to bond to the enzymes or microorganism by standing to give the objective biocatalyst A immobilized on the crosslinked films 3.