| 摘要 |
PURPOSE:To obtain a plasmid useful as a vector for recombinant gene technique, by forming plasmid pSM1 (0021) having 3,8 megadalton molecular weight, shown by a specific restricted enzyme cleavage map. CONSTITUTION:Saccharomyces montanus IFO0021 is cultivated in a liquid medium. A nutritive medium containing a carbon source and a nitrogen source is usable as the liquid medium, glucose can be used as the carbon source, and polypeptone, yeast extract, etc., as the nitrogen source. The fittest growth of Saccharomyces montanus is attained usually in about 1-3 days, and, when the culture is carried out in a large culture tank, a former culture solution is preferably prepared. A culture mold from it is subjected to bacteriolysis, and plasmid pSM1 (0021) is separated from the bacteriolysis substance. The plasmid is characterized by a restricted enzyme cleavage map shown by the figure. |
