L-glutamic acid oxidase (H2O2-generating), its production and analytical method therefor

摘要

L-glutamic acid oxidase having the following biochemical properties: (a) substrate specificity: L-glutamic acid, (b) enzyme action: catalyzes a reaction which forms one mole of alpha -ketoglutaric acid, one mole of ammonia and one mole of hydrogen peroxide from one mole of L-glutamic acid, one mole of oxygen and one mole of water, as follows: [I] This oxidase is produced by culturing microorganisms belonging to genus Streptomyces in a nutrient medium and isolating the thus-produced L-glutamic acid oxidase. Particular microorganisms of genus Streptomyces are sp. A7700 FERM P-6241 (NRRL No. 15267) and sp. 8063 FERM P-6242 (NRRL No. 15268). The oxidase can be used for detecting L-glutamic acid or L-glutamate, in an aqueous sample, because it catalyzes a reaction which forms one mole of alpha -ketoglutaric acid, one mole of ammonia and one mole of hydrogen peroxide from one mole of glutamic acid, one mole of oxygen and one mole of water. Thus, the consumed oxygen can be quantitatively determined, or the generated alpha -glutaric acid or ammonia or hydrogen peroxide can be quantitatively determined.