| 摘要 |
PURPOSE:To produce a D-alpha-amino acid easily in high yield, by reacting a D-5- substituted hydantoin with a microorganism of the genus Hansenula having a special ability. CONSTITUTION:About 30g/l microbial cells of Hansenula polymorpha washed with physiological saline solution is added to a D-5-substituted hydantoin expressed by formula I [R is (substituted) alkyl, (substituted) aralkyl, (substituted) phenyl, furyl, pyridyl, thiazolyl, imidazolyl or indolyl], e.g. D-5- methylhydantoin, as an enzymic reaction substrate adjusted to 0.1-10wt% concentration, and reacted and converted therewith at 20-60 deg.C and 5-9pH for about 20hr. The resultant D-alpha-amino acid is then passed through steps of concentration, neutralization and ion exchange resin treatment to give the aimed D-alpha-amino acid expressed by formula II (R is same as formula I).
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