| 摘要 |
Immunochemical quantitative determination of antigenically active cpds. (A) comprises (1) incubating (A) with a binder (B1; i.e. a labelled specific antibody or its Fab or Fab' fragment); (2) incubating the reaction mixt. for a specified time with solid phase (A) or analogue; (3) sepn. of liq. phase and (4) opt. washing the solid and combining the washings with the liq. phase. - The new feature is that the liq. phase is then reacted with a solid-phase second antibody (B2) which is specific for (B1) (or for its complex with (A), provided that in this case it is not cross-reactive with any of the complex components). The solid phase is sepd., opt. washed and the amt. of label measured. - Also new is a reagent consisting of (1) B1; (2) solid-phase (A); (3) solid-phase B2 and (4) system for measuring the label.
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