CARRIER-BOUND HISTAMINE, ITS MANUFACTURE AND ITS USE

摘要

Carrier-bound histamine comprising histamine in the form of an aliphatically bound 4-(2-aminoethyl)-imidazolyl group. Process for binding histamine covalently to a carrier containing at least one functional group Y, in that a histamine derivative of formula (I) in which R1NH is a group which is chemically inert to X and Y, with R1 being hydrogen or a protective group capable of being split off; n is an integer of less than 10; m is an integer of 0 or 1; B is an inert organic bridge; and X and Y are functional groups such that X and Y can be reacted chemically with each other to form a covalent structure binding together said histamine derivative and said carrier; is contacted with said carrier so that X and Y are reacted with each other to covalently bind said derivative and carrier together, whereupon, if R1 is other than hydrogen, R1 is converted chemically to hydrogen. The carrier-bound histamine can be used for chromatography and in immunological assay methods.