发明名称 |
INTACT GENE AND METHOD OF EXCISING AND CLONING SAME |
摘要 |
The present invention discloses isolated functionally intact whole genes and method of obtaining the same. The method includes treating genomic DNA with a nuclease and a denaturing agent under controlled conditions. The invention also discloses cloning of said intact whole genes and a library of such cloned genes or any recombinations thereof. The invention is useful in deriving gene products such as m-RNA, S-RNA, t-RNA, polypeptides and the like. |
申请公布号 |
ZA8505023(B) |
申请公布日期 |
1986.07.30 |
申请号 |
ZA19850005023 |
申请日期 |
1985.07.03 |
申请人 |
THE UNITED STATES OF AMERICA, REPRESENTED BY THE SECRETARY U.S. DEPARTMENT OF COMMERCE |
发明人 |
THOMAS F. MCCUTCHAN;JOHN BARTON DAME |
分类号 |
G01N33/50;C07K14/445;C12N15/00;C12N15/09;C12N15/10;C12Q1/68;C12R1/01;G01N33/53 |
主分类号 |
G01N33/50 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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