| 摘要 |
PURPOSE:To obtain the titled aqueous solution, by removing a protein denaturation agent from a dilute aqueous solution of human gamma-type interferon containing a protein denaturation agent, aging the solution in dissolved state, and concentrating the aged solution. CONSTITUTION:A dilute aqueous solution of human IFN-(R) (e.g. an aqueous solution of human IFN-(R) monomer obtained by the gel-filtration of crude human IFN-(R) in the presence of a reducing sulfur compound and a protein denaturation agent) containing a protein denaturation agent (e.g. guanidine salt, urea, thiocyanate, etc.) is subjected to the gel-filtration or ultrafiltration to remove the protein denaturation agent. The obtained dilute aqueous solution is let stand in liquid state at 0-40 deg.C for 0.5-7 days, preferably for 24-72hr to effect the aging of the solution. If necessary, the solution is subjected to e.g. aseptic filtration, etc. before aging. The aged solution is concentrated with an ultrafiltration membrane (having an MWCO of about 10,000) to obtain an aqueous solution of highly concentrated human IFN-gamma containing 0.2-1.5mg/ml of human IFN-(R). The product is suitable as an intermediate for the mass production of human IFN-gamma.
|
