| 摘要 |
A hybridization method for the identification of nucleic acids in solution is presented. At least two probes, a detector probe and a capturing probe are used in the method. The detector probe is labelled with a detectable label and to the capturing probe a member of an affinity pair is attached. The capturing probe: target nucleic acid: detector probe hybrid formed in the hybridization reaction is isolated by the aid of the other member of the affinity pair. Suitable affinity pairs are biotin-avidin or biotin-streptavidin homopolynucleotides, antigens-antibodies etc. The method is faster to perform than the traditional sandwich hybridization. |
