| 摘要 |
PURPOSE:To obtain the titled membrane having uniform membrane thickness simply and surely even if the surface of solid surface is large, by sticking the flat solid surface fast to a thin membrane of enzyme solution spread on a frame, and sticking the solid surface having formed the enzyme membrane fast to a thin membrane of a solution of a polyfunctional reagent spread on a frame. CONSTITUTION:The structure 1 is immersed in a solution of an enzyme or it and protein, and slowly pulled up to form the thin membrane 2. The surface of the flat solid 3 such as plate glass, etc., is pressed against the thin membrane 2 and stuck fast to it. Then, the surface of the solid having formed the enzyme membrane is pressed against a thin membrane of a polyfunctional reagent such as glutaraldehyde, etc. or it and a surface active agent spread on another frame structure, stuck fast to it, and a crosslinking reaction is carried out, to give an enzyme immobilized membrane. The enzyme immobilized membrane having uniform membrane thickness simply and surely even if the area of the solid surface is large is formed on the surface of the solid.
|
