| 摘要 |
<p>A composition of matter, namely the nucleotide sequence of the DNA gene encoding yeast premelibiase and the amino acid sequence so contained in the premelibiase protein. This invention discloses the DNA sequence of the premelibiase gene, together with the amino acid sequence of the secreted melibiase and so defines the amino acid sequence of the pre-region, and the nucleotide sequence that encodes this sequence. The pre-region of premelibiase can be used in engineering secretion, from genetically-modified yeast, of peptides and proteins other than melibiase, since fusions made between its nucleotide sequence and that coding for foreign peptides and proteins will ensure secretion of these foreign gene products in yeast, when contained in appropriate gene expression vehicles. Also disclosed is the nucleotide sequence of the promoter region of the premelibiase gene, this sequence being regulatable by protein products of the genes GAL4 and GAL80. The promoter region may be integrated into a vector construct along with a structural gene and optionally including the signal sequence defined above in order to obtain controllable expression of a protein in a transformed yeast host.</p> |
