| 摘要 |
PURPOSE:To enable rapid measurement, by constituting the titled membrane so that the membrane contacted with a measuring specimen side is formed of a hydrophobic porous membrane having fine pores with a pore size of 0.01-0.06mum and the membrane contacted with an electrode side is formed of a cellulose acetate membrane with a thickness of about 4-6mum while the activity of the enzyme membrane immobilized between both membranes is set to 0.001-0.009U/cm<2>. CONSTITUTION:An immobilized enzyme membrane is constituted by laminating an immobilized enzyme layer 7 by two kinds of porous membranes 6, 8 and the polycarbonate membrane 8 contacted with a specimen side comprises a porous hydrophobic membrane such as a reverse osmosis membrane or ultrafiltration membrane made of polycarbonate and has fine pores with a pore size of 0.01-0.06mum. The cellulose acetate membrane 6 contacted with an electrode side pref. has a thickness of about 4-6mum. The immobilized enzyme membrane has an immobilized enzyme layer 7 between two kinds of the cellulose acetate membrane 6 and the polycarbonate membrane 8 and the activity of the immobilized enzyme layer is adjusted to a range of 0.001-0.009U/cm<2>. |
