摘要 |
PURPOSE:To easily execute a desired analysis by bringing a labeling antigen (antibody) and antigen (antibody) in a sample into reaction then separating the same to the immune complex and the remaining antigen (antibody) (B-F sepn.) and measuring the same with a flow sight meter. CONSTITUTION:A latex soln. conjugated with a solid phase antibody, sample and labeling antibody soln. are added by a sample drawing device 1 and a reagent injector 2 into a reaction vessel 3 contg. a buffer soln. for reaction and the reactive liquid from the cell 3 is subjected to the B-F sepn. in a separator 4. The immune complex remaining by failing to pass through the separator 4 is diluted by the buffer soln. of a diluting device 6. The soln. is introduced into a flow cell 7 and light 8 is irradiated on the soln. The size of the immune complex and the fluorescence quantity /1 latex riding thereon are measured by a data processing unit 11 and the sight gram is obtd. from these two parameters. The analytical result having the high quantitative determination characteristic is thus obtd. simply by introducing the sample into the flow system. |