DETERMINATION OF PREDATION CAPACITY OF BIOPHAGOUS CELL

摘要

PURPOSE:To simply and accurately measure the predation capacity of a biophagous cell with good sensitivity, by allowing the biophagous cell to take in a fluorescence labelled particle or microorganism and subsequently separating the biophagous cell to dissolve the same while measuring the fluorescent intensity of the obtained solution. CONSTITUTION:A fluorescent substance such as fluorescamine or fluorescein isothiocyanate is reacted with a fine particle pref. porous and having a particle size of 20mum or less such as one prepared by introducing an amino group into an inorg. particle such as glass or silica or an org. polymer particle having an amino group to obtain a fluorescence labelled particle. Bacteria or yeast is labelled by the fluorescent substance in the same way. Either one of the aforementioned labelled ones is added to a culture solution, obtained after a biophageous cell such as microphage or neutrophil separated from the living body was cultured, in a definite time to perform inculation for a definite time. The biophagous cell is separated and the adhered labelled substance is removed by washing while the separated biophagous cell is dissolved in a bacteolytic solution and the fluorescent intensity of the obtained solution is measured. By using a preliminarily calculated calibration curve, the predation capacity of the biophagous cell is accurately known.