| 摘要 |
<p>Proteinaceous materials extracted from pyogenic bacteria, streptococci, staphylococci and Klebsiella species, act as specific inhibitors of human luekocyte elastase and may be used to treat deseases which include elastolysis as part of their desease processes e.g., pulmonary emphysema, adult respiratory distress syndrome and bacterial pneumonites. The proteinaceous materials extracted from the pyogenic bacteria can be conveniently purified by providing an affinity column by various novel methods such as the following five step methods: (1) binding elastin to an affinity column support material (2) binding human leukocyte elastase to the elastin (3) binding the human leukocyte elastase inhibitors to the elastase (4) eluting the affinity column with a first elution medium capable of separating contaminating substances from the inhibitors and (5) separating the inhibitors from the elastase by use of a second elution medium having a raised salt concentration.</p> |
