发明名称 |
IMMOBILIZED METAL ION AFFINITY CHROMATOGRAPHY PROTEIN PURIFICATION |
摘要 |
This invention describes a process for separating a biologically active polypeptide or protein in the form of its precursor from a mixture containing said precursor and impurities, which comprises contacting said precursor with a resin containing immobilized metal ions, said precursor comprising the biologically active polypeptide or protein covalently linked directly or indirectly to an immobilized metal ion chelating peptide, binding said precursor to said resin, and selectively eluting said precursor from said resin. Such precursor compounds are also described. |
申请公布号 |
AU5024085(A) |
申请公布日期 |
1986.06.12 |
申请号 |
AU19850050240 |
申请日期 |
1985.11.21 |
申请人 |
LILLY ELI AND CO. |
发明人 |
MICHELE CECEIL SMITH;CHARLES PIDGEON |
分类号 |
C12N15/09;A61K35/74;A61K38/00;A61K38/16;A61K38/27;A61K38/28;A61K38/43;A61K38/44;A61K38/46;A61K38/55;A61K39/00;B01J20/32;C07K1/16;C07K1/22;C07K14/575;C07K14/61;C07K14/62;C07K17/14;C07K19/00;C12N15/00;C12N15/62;C12P21/00 |
主分类号 |
C12N15/09 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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