METHOD AND INSTRUMENT FOR OPERATING COLUMN CHROMATOGRAPHY

摘要

PURPOSE:To execute automatically the detection and quantitative determination of the component contained in an eluate and the measurement of the active value thereof by measuring continuously the quantity of the effective component contained in the eluate. CONSTITUTION:The eluate in a storage task 2 is rested for a short period and is heated to a prescribed temp.; thereafter, a specified amt. of the eluate is sampled by a micro-syringe 4 and is injected and added to a mixing tank 12 by the movement of the syringe 4. The substrate liquid to be measured is preliminarily conducted at a specified rate from a substrate liquid tank 5 into the tank 12 by a micro-metering pump 8 through an air venting device 6 and a substrate selector valve 7. The liquid is stirred by a mixing device 13 when the eluate is added to the substrate liquid in the tank 12 and immediately the mixture is conducted by a micro-metering pump 18 into and absorptivity measuring instrument 15. The reactive liquid of the substrate liquid and the eluate stays for a specified period in the instrument 15. The change of the absorptivity value during this time is fed to a CPU16. The active value is immediately calculated in this stage and whether oxygen fraction or unnecessary fraction is judged in accordance with the active value.