| 摘要 |
PURPOSE:To decrease the heterologous interference of heteroantibodies, and to purify the antibody to the one suitable especially for the low-concentration immunoglobulin assay, by immobilizing an antibody of an antibody fluid col lected from two kinds of animals to an insoluble carrier, and contacting the immobilized antibody with the other antibody liquid. CONSTITUTION:In an immunoassay using a sandwich process wherein the antigen separated from the first animal species is sandwiched stepwise with antibodies collected from the second and the third animal species, the antibody is purified by immobilizing either the second or the third antibody to an insoluble carrier (e.g. CNBR-activated sephalose column), and contacting the antibody to the other antibody. The reactive components in the heteroantibodies can be removed, and the mutual interference can be decreased. Accordingly, an immunoassay can be carried out in high sensitivity. It is especially useful for the purification of an antibody suitable for the assay of a low-concentration immunoglobulin.
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