| 摘要 |
PURPOSE:To obtain a biosensor with which stable performance is obtd. without exfoliation, etc. of an immobilized enzyme film, etc. for a long period of time by forming a metallic hydroxide gel film immobilized with enzyme on the sensitive surface of an electrode sensitive with chemical materials in tight contact therewith. CONSTITUTION:A soln. prepd. by adding an acid to a mixed water-alcohol solvent of metallic alkoxide such as Si(OCH3)4 or Ti(OC3H7)4 is coated on the sensitive surface 3 at the top end of a cylindrical pH glass electrode 2 contg., for example, an Ag/AgCl internal electrode and an internal liquid 22 and is dried to form the metallic hydroxide gel film thereon. The required enzyme is then bound with the gel film via a silane coupling agent to form the immobilized enzyme film 4. The film 4 is otherwise formed on the gate 55 of an MOS field effect type transistor element (pH-ISFET)5 in the same manner as mentioned above. The biosensors 1, 1' which have the substantial adhesiveness between the film-like carrier part and the sensitive surface, obviate the exfoliation, etc. even after long-term repetitive use and can maintain the stable performance are thus obtd. |
