| 摘要 |
<p>PURPOSE:In the production of plasminogen activator precursor, neutral aminoacids and organic acids or their salts are added to increase the production of the plasminogen activator precursor. CONSTITUTION:As cells for producing plasminogen activator precursors, human renal cells are suitable and the cells which have been obtained after subculture of the primary culture from human renal cells. Glycine, alanine, leucine and other neutral aminoacids and organic acid such as carboxylic acids or their salts are added to effect the culture of cells which produce plasminogen activator precursor. The culture medium is exchanged and the precursor is collected from the exchanged medium by centrifugation, gel filtration and other operations.</p> |
