| 摘要 |
NEW MATERIAL:A bacterial strain RS-0001 (FERM P-7918) belonging to Clostridium genus. USE:A bacterial strain capable of producing a novel heat-resistant alpha-amylase, and having excellent heat-resistance, extremely low calcium-requirement, and high activity even under acidic condition. PREPARATION:A high-temperature methane-fermentation slurry is centrifuged at a low speed to remove coarse particles, and the slurry is diluted with sterilized physiological saline water to obtain a bacterial cell dispersion. The dispersion is cultured anaerobically at 60 deg.C on an agar plate containing starch grains as carbon source, the colony is separated, a vegetative cell is separated from the diluted suspension of the colony with a micromanipulator, and the above separation procedures are repeated to obtain the objective bacterial strain. |
