| 摘要 |
PURPOSE:To reduce load of desalting of starch processing, to prevent disadvanta geous isomerization of reduction end, and to liquefy starch at low viscosity, by using thermostable alpha-amylase having calcium demanding properties with necessary calcium concentration of <= specific value in thermostability. CONSTITUTION:A thermostable anaerobic bacterium belonging to the genus Clostridium is cultivated in a mold, centrifuged to remove a mold, the superna tant liquid is further filtered a molecular sieve membrane, etc., and concentrated. Then, it is passed through a column packed with a crosslinked dextran, subjected to liquid chromatography, so that thermostable alpha-amylase having calcium demanding properties of <=10muM necessary calcium concentration in thermostability is separated. Starch (e.g., potato starch, etc.) is treated with this thermostable alpha-amylase at <=5.5pH, and liquefied.
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