| 摘要 |
Excretable beta-galactosidase (I) isolated from other excreted prods. is new. DNA fragment comprising a gene naturally present in Streptomyces sp. and coding for an excretable (I) is new. DNA fragment comprising a gene coding for the Bgl protein, the gene being naturally present in Streptomyces sp. immediately upstream of a gene coding for an excretable (I) is new. - The gene is pref. naturally present in Strep. lividans 1326 chromosomal DNA. It is pref. carried on a 2.20 kb Bal I-Bgl II fragment mapped with Bal I at 1.19, Stu I at 1.37, Sal I at 1.70, Bcl I at 2.10, Bgl II 2.47 and Bgl II at 3.39 kb. DNA fragment comprising the Streptomyces (I) promoter is new. Micro-organism transformed with a DNA fragment above is new. - Prepn. of a DNA fragment carrying a gene coding for an excretable (I) comprises isolation of the gene from a strain of Streptomyces sp. producing (I). Assay of gene expression comprises transformation of a micro-organism with the DNA fragment defined in para. d(B),dfol(B) followed by the determn. of excreted (I) with a chromogenic substrate not readily taken up by host cells. (0/3) DEAB- DE3586652 G Excretable beta-galactosidase (I) isolated from other excreted prods. is new. - DNA fragment comprising a gene naturally present in Streptomyces sp. and coding for an excretable (I) is new. - DNA fragment comprising a gene coding for the Bgl protein, the gene being naturally present in Streptomyces sp. immediately upstream of a gene coding for an excretable (I) is new. The gene is pref. naturally present in Strep. lividans 1326 chromosomal DNA. - It is pref. carried on a 2.20 kb Bal I-Bgl II fragment mapped with Bal I at 1.19, Stu I at 1.37, Sal I at 1.70, Bcl I at 2.10, Bgl II 2.47 and Bgl II at 3.39 kb. - DNA fragment comprising the Streptomyces (I) promoter is new. Micro-organism transformed with a DNA fragment above is new. Prepn. of a DNA fragment carrying a gene coding for an excretable (I) comprises isolation of the gene from a strain of Streptomyces sp. producing (I). - Assay of gene expression comprises transformation of a micro-organism with the DNA fragment defined in para. d(B),dfol(B) followed by the determn. of excreted (I) with a chromogenic substrate not readily taken up by host cells. EPAB- EP-187630 B A recombinant DNA molecule comprising a DNA fragment containing the promoter of the Streptomyces beta-galactosidase gene, not linked to the beta-galactosidase structural gene, which promoter is located in the Pst(8.8)-Xmnl(9.9)region of the 7 kb Pstl-Sphl region of Streptomyces lividans strain 1326 chromosomal DNA. (Dwg.0/1) |
