| 摘要 |
PURPOSE:To obtain economical manufacturing method of a biosensor characterized by a small amount of consumption of enzyme, by forming a plurality of ion sensitive parts on a chip, coating a part other than the sensitive parts by a hydrophobic resin beforehand, holding a liquid including the enzyme, and forming an immobilized enzyme film. CONSTITUTION:A part on a substrate 1 other than ion sensitive parts 2 is coated by a hydrophobic resin 3. A solution including enzyme 4 having albumin and enzyme is formed on one gate by a syringe. The solution including enzyme 4 loses water and a film including enzyme 5 is formed in several minutes. Then glutaraldehyde is supplied thereon as a bringing agent 6. It is repelled by a resin layer (fluoride) and held on the previously formed protein film. This is made to remain for several minutes. Then the protein bridge is favorably expanded, and a fixed enzyme film 7 is formed. Then the product is made to remain intact for a specified time period. After washing with water and glycine treatment, the product is stored in a buffer. Thus a small amount of the enzyme can be well fixed on a minute sensor. |
