METHOD OF PRODUCING IMMOBILIZED AMINOACYLASE

摘要

The invention is concerned with a process for the immobilization of an aminoacylase enzyme by activating a partially hydrolyzed copolymer, prepared by subjecting an acrylamide/N,N min -methylene-bis-(acrylamide) copolymer optionally alkaline, hydrolysis and applying to it the aminoacylase, in which a partially hydrolyzed acrylamide/N,N min -methylene-bis-acrylamide) copolymer which may be prepared beyond by alkaline hydrolysis alternatively by acidic hydrolysis is treated in a manner known per se with a carbodiimide compound which is soluble in water or soluble in an organic solvent at a temperature below 0 DEG C, a solution of the aminoacylase with a pH of 6.5 to 8.5 is applied onto the resulting activated support and in a manner known per se the resulting product is washed and then, if desired, dried. The so obtained immooilized enzyme preparations have an extremely high specific activity.