发明名称 |
CLONING OF MODIFIED STREPTOMYCES DNA |
摘要 |
A method of cloning endogenously modified Streptomycetes DNA, which is normally rejected by restrictionless heterospecific hosts, is disclosed. The method uses bacteriophage lambda to construct a genomic library of modified Streptomycetes DNA; such lambda-containing Streptomycetes DNA is replicated to provide a source of non-modified Streptomycetes DNA. This non-modified DNA is subcloned into a selectable cloning vector and used to transform restrictionless hetero-specific hosts. The transformants can then be screened for clones containing genes of interest. |
申请公布号 |
JPS6185189(A) |
申请公布日期 |
1986.04.30 |
申请号 |
JP19850213601 |
申请日期 |
1985.09.25 |
申请人 |
ELI LILLY & CO |
发明人 |
CHIYAARUZU RII HAASHIYUBAAGAA;JIEFURII RIN RAASON |
分类号 |
C12N15/09;C12N1/20;C12N15/63;C12N15/76;C12R1/19;C12R1/465 |
主分类号 |
C12N15/09 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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