| 摘要 |
PURPOSE:To measure a substance possessing an antigen determining group with high sensitivity, by using enzyme capable of acting on a water-insoluble high-molecular substance. CONSTITUTION:A ligand being a substance possessing two or more of antigen determining groups contained in a specimen is contacted and reacted with a combined substance of the antigen to one antigen determining group of said ligand and enzyme capable of acting on a water-insoluble high-molecular substance, and the antigen to the other antigen determining group. Further, the aforementioned combined substance is contacted with the aforementioned high- molecular substance to perform enzymatic reaction and enzymatic activity is measured to determine the substance possessing the antigen determining groups. The enzyme is one capable of acting on the water-insoluble high- molecular compound and the bonding method of the antigen may be determined with due regard to both functional groups. The antibody acting on the ligand is one reacted with the antigen determining group different from the antigen determining group reacted with the antigen of the combined substance. |
