| 摘要 |
PURPOSE:To enhance a treatment speed, in the measurement of immune reaction according to a rate method using nephelometry, by estimating the max. value of a reaction speed without performing continuous photometry. CONSTITUTION:The curve showing the relation between the concn. of the antigen-antibody composite substance and reaction time in antigen-antibody reaction resembles to the curve at the central part of a cubic curve as shown by the drawing. A reaction reagent is successively added to a plurality of specimens to react an antigen and an antibody while the intensity of scattered light is measured in the addition order of the reagent and, when measurement was finished once, the intensity of scattered light is repeatedly measured from the first specimen in the same way and this measurement is performed at least three times with respect to each specimen to calculate the cubic expression, that is, constants (a), (b), (c) of formula I (wherein I is the intensity of scattered light and T is reaction elapse time) with respect to each specimen. These constants are substituted for formula II to calculate the max. reaction speed in the antigen/antibody between the specimen and the reagent. |
