摘要 |
An antigen-independent immunoassay system based on antibodies (a-Ag) to a given antigen (Ag) uses anti-idiotype antibodies (a-Id) to the a-Ag antibodies as a reference component in place of purified antigen. The a-Id may be monoclonal and produced by fusing lymphocytes, from an animal in which has been grown a hybridoma tumour secreting a-Ag, with myeloma cells and then isolating and cloning hybridoma cells secreting a-Id. |