发明名称 |
Membrane immune assay |
摘要 |
An immunoassay method for detection of antigen is disclosed. The method employs complement mediated lysis of vesicles loaded with In-111 or other gamma-emitting cation, and quantitative detection of the lysis by gamma-ray perturbed angular correlation (PAC) spectroscopy. The vesicles are labeled with a substance competitive to the antigen to be measured, and the concentration of the antigen in the sample measured by assessing the diminution in lysis due to the presence of the competing antigen. The method may also be used to assess the immunologic competence of a subject by injecting suitably sensitized vesicles and monitoring the in vivo lysis pattern by (PAC).
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申请公布号 |
US4581222(A) |
申请公布日期 |
1986.04.08 |
申请号 |
US19830465360 |
申请日期 |
1983.02.09 |
申请人 |
CALIFORNIA INSTITUTE OF TECHNOLOGY |
发明人 |
BALDESCHWIELER, JOHN D.;GAMBLE, RONALD C.;LIN, ALBERT M.;TIN, GEORGE W. |
分类号 |
G01N33/534;(IPC1-7):A61K43/00;G01T1/00 |
主分类号 |
G01N33/534 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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